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Dr. James R. Heath is President and Professor at Institute for Systems Biology in Seattle. Heath also has Affiliate Faculty appointments at the University of Washington Bioengineering, Physics and Chemistry departments. Formerly, he was the Elizabeth W. Gilloon Professor of Chemistry at Caltech, Professor of Molecular and Medical Pharmacology at UCLA, and served as co-director of the Parker Institute for Cancer Immunotherapy at UCLA.

Heath has had a profound impact on ISB since taking over leadership in 2018. He continues to make important discoveries in the field of cancer immunotherapy, and in 2022 was selected to lead a National Cancer Institute Comprehensive Cancer Center to study sequential targeted inhibitors and immunotherapies.

At the beginning of the COVID-19 pandemic, Heath quickly pivoted the focus of his lab and collaboratively worked with others within and beyond ISB to uncover secrets behind COVID-19 and long COVID. He published research that has changed how COVID-19 and long COVID is understood, and leads the Pacific Northwest consortium of the NIH-funded RECOVER study, which aims to understand the long-term effects of COVID.

Heath received his PhD in 1988 from Rice University, where he was the principal graduate student involved in the discovery of C60 and the fullerenes. He was a Miller Fellow at UC Berkeley before joining the research staff at IBM Watson Labs in 1991. He took a faculty position at UCLA in 1994, and moved to Caltech in 2003.

He has received several awards and honors, including the Irving Weinstein Award from the American Association of Cancer Researchers, and the Sackler Prize in the Physical Sciences. In 2009, he was named one of the top seven innovators in the world by Forbes Magazine.

Heath has founded or co-founded several companies, including BioAnalytica, PACT Pharma (acquired by AmplifyBio in 2023), Integrated Diagnostics (acquired by Biodesix in 2018), Indi Molecular (acquired by Regeneron in 2022), CTI Molecular Imaging (acquired by Siemens in 2005), Sofie Biosciences, Isoplexis (acquired by Berkeley Lights in 2023), and NanoSys (acquired by Shoei in 2023).

2018 – Present
President and Professor, Institute for Systems Biology, Seattle

2003 – 2018  
Elizabeth W. Gilloon Professor & Professor of Chemistry, Caltech

2001 – Present
Professor of Molecular & Medical Pharmacology, UCLA Health Sciences Center

1997 – 2002    
Prof., UCLA;   1996-97, Assoc. Prof., UCLA;   1994-96, Asst. Prof., UCLA

1991 – 94  
Research Staff, IBM T.J. Watson Res. Labs, Yorktown, NY

1988 – 1991  
UC Berkeley Miller Fellowship, Postdoctoral Scholar

1988   
Rice University, PhD, Chemistry

1988   
Rice University, MA, Chemistry

1984    
Baylor University, BSc, Chemistry

2323737 2RQKSFR5 james r. heath 1 chicago-author-date-16th-edition 50 date desc year 1 1 7373 https://isbscience.org/wp-content/plugins/zotpress/
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Horwitz, Leora I., Jacqueline H. Becker, Weixing Huang, Teresa Akintonwa, Maxwell M. Hornig-Rohan, Gabrielle Maranga, Dara R. Adams, et al. 2025. “Olfactory Dysfunction After SARS-CoV-2 Infection in the RECOVER Adult Cohort.” JAMA Network Open 8 (9): e2533815. http://doi.org/10.1001/jamanetworkopen.2025.33815. Cite Download
Qualls, Anita E., Tasha Tsao, Irene Lui, Shion A. Lim, Yapeng Su, Ernie Chen, Dylan Duchen, et al. 2025. “High Affinity CD16 Polymorphism Associated with Reduced Risk of Severe COVID-19.” JCI Insight, e191314. http://doi.org/10.1172/jci.insight.191314. Cite
Snyder, Thomas M., Rachel M. Gittelman, Mark Klinger, Damon H. May, Edward J. Osborne, Ruth Taniguchi, H. Jabran Zahid, et al. 2025. “Magnitude and Dynamics of the T-Cell Response to SARS-CoV-2 Infection at Both Individual and Population Levels.” Frontiers in Immunology 15: 1488860. http://doi.org/10.3389/fimmu.2024.1488860. Cite
Armstrong, Sujit Silas, Daniel G. Chen, Sunil Kumar, James R. Heath, Matthew J. Feinstein, John R. Greenland, Daniel R. Calabrese, Lewis L. Lanier, Klaus Ley, and Avishai Shemesh. 2025. “CITE-Seq Analysis Reveals a Differential Natural Killer Cell SPON2 Expression in Cardiovascular Disease Patients Impacted by Human-Cytomegalovirus Serostatus and Diabetes.” International Journal of Molecular Sciences 26 (3): 1369. http://doi.org/10.3390/ijms26031369. Cite Download
Geng, Linda N., Kristine M. Erlandson, Mady Hornig, Rebecca Letts, Caitlin Selvaggi, Hassan Ashktorab, Ornina Atieh, et al. 2025. “2024 Update of the RECOVER-Adult Long COVID Research Index.” JAMA. http://doi.org/10.1001/jama.2024.24184. Cite
Heath, James R., and RECOVERY Collaborative Group. 2025. “Higher Dose Corticosteroids in Patients Admitted to Hospital with COVID-19 Who Are Hypoxic but Not Requiring Ventilatory Support (RECOVERY): A Randomised, Controlled, Open-Label, Platform Trial.” Lancet (London, England) 401 (10387): 1499–1507. http://doi.org/10.1016/S0140-6736(23)00510-X. Cite Download
Nolan, Sean, Marissa Vignali, Mark Klinger, Jennifer N. Dines, Ian M. Kaplan, Emily Svejnoha, Tracy Craft, et al. 2025. “A Large-Scale Database of T-Cell Receptor Beta Sequences and Binding Associations from Natural and Synthetic Exposure to SARS-CoV-2.” Frontiers in Immunology 16: 1488851. http://doi.org/10.3389/fimmu.2025.1488851. Cite Download
Tripathi, Dhiraj, Kelly Handley, Lisa Holden, Zainab Abdali, Sue Jowett, Jonathan Mathers, Christopher Poyner, et al. 2025. “Clinical Trial: A Multicentre Randomised Controlled Trial of Carvedilol Versus Variceal Band Ligation in Primary Prevention of Variceal Bleeding in Liver Cirrhosis (CALIBRE Trial).” Alimentary Pharmacology & Therapeutics. http://doi.org/10.1111/apt.70080. Cite
Flower, Cameron T., Chunmei Liu, Hui-Yu Chuang, Xiaoyang Ye, Hanjun Cheng, James R. Heath, Wei Wei, and Forest M. White. 2025. “Signaling and Transcriptional Dynamics Underlying Early Adaptation to Oncogenic BRAF Inhibition.” Cell Systems, 101239. http://doi.org/10.1016/j.cels.2025.101239. Cite
Zaslavsky, Maxim E., Erin Craig, Jackson K. Michuda, Nidhi Sehgal, Nikhil Ram-Mohan, Ji-Yeun Lee, Khoa D. Nguyen, et al. 2025. “Disease Diagnostics Using Machine Learning of B Cell and T Cell Receptor Sequences.” Science (New York, N.Y.) 387 (6736): eadp2407. http://doi.org/10.1126/science.adp2407. Cite
Xie, Jingyi, Daniel G. Chen, William Chour, Rachel H. Ng, Rongyu Zhang, Dan Yuan, Jongchan Choi, et al. 2025. “APMAT Analysis Reveals the Association between CD8 T Cell Receptors, Cognate Antigen, and T Cell Phenotype and Persistence.” Nature Communications 16 (1): 1402. http://doi.org/10.1038/s41467-025-56659-3. Cite
Shah, Dimpy P., Tanayott Thaweethai, Elizabeth W. Karlson, Hector Bonilla, Benjamin D. Horne, Janet M. Mullington, Juan P. Wisnivesky, et al. 2025. “Sex Differences in Long COVID.” JAMA Network Open 8 (1): e2455430. http://doi.org/10.1001/jamanetworkopen.2024.55430. Cite Download
Ng, Rachel H., Dan Yuan, Rongyu Zhang, Sarah Li, Lauren Jatt, Rick Edmark, Inyoul Lee, et al. 2024. “174 Longitudinal Multiomic Analysis Associates T Cell Dynamics with Chemotherapy Outcome in Ovarian Cancer Patients.” Journal for ImmunoTherapy of Cancer 12 (Suppl 2). http://doi.org/10.1136/jitc-2024-SITC2024.0174. Cite Download
Zhang, Rongyu, Rachel H. Ng, Michaela McKasson, William Chour, Andrew Webster, Jongchan Choi, Jingyi Xie, Cornelia Trimble, Antoni Ribas, and James R. Heath. 2024. “406 High Throughput Identification of Cancer-Specific CD4+ T Cells for TCR T-Cell Immunotherapy through Engineered Proteins.” Journal for ImmunoTherapy of Cancer 12 (Suppl 2). http://doi.org/10.1136/jitc-2024-SITC2024.0406. Cite Download
Zhang, Rongyu, Rachel Ng, Michaela McKasson, William Chour, Andrew Webster, Jongchan Choi, Jingyi Xie, Cornelia Trimble, Antoni Ribas, and James R. Heath. 2024. “Identifying Cancer-Specific T Cells for TCR T-Cell Immunotherapy through Engineered Proteins.” The Journal of Immunology 212 (1_Supplement): 1400_5907. http://doi.org/10.4049/jimmunol.212.supp.1400.5907. Cite Download
Swank, Zoe, Ella Borberg, Yulu Chen, Yasmeen Senussi, Sujata Chalise, Zachary Manickas-Hill, Xu G. Yu, et al. 2024. “Measurement of Circulating Viral Antigens Post-SARS-CoV-2 Infection in a Multicohort Study.” Clinical Microbiology and Infection. http://doi.org/10.1016/j.cmi.2024.09.001. Cite
Ng, Rachel, Dan Yuan, Inyoul Lee, Sarah Li, Pamela Troisch, Sergey Kornilov, Brett Smitt, et al. 2024. “Longitudinal Multiomic Analysis Associates T Cell Dynamics with Recurrence of Ovarian Cancer after Chemotherapy.” The Journal of Immunology 212 (1_Supplement): 0456_5804. http://doi.org/10.4049/jimmunol.212.supp.0456.5804. Cite Download
Marchal, Astrid, James R. Heath, Elizabeth T Cirulli, Iva Neveux, Evangelos Bellos, Ryan S. Thwaites, Yu Zhang, and The COVID Study Group. 2024. “Lack of Association between Classical HLA Genes and Asymptomatic SARS-CoV-2 Infection.” Human Genetics and Genomics Advances 5 (3). http://doi.org/10.1016/j.xhgg.2024.100300. Cite Download
Flower, Cameron T., Chunmei Liu, Hui-Yu Chuang, Xiaoyang Ye, Hanjun Cheng, James R. Heath, Wei Wei, and Forest M. White. 2024. “Signaling and Transcriptional Dynamics Underlying Early Adaptation to Oncogenic BRAF Inhibition.” bioRxiv, 2024–02. https://www.biorxiv.org/content/10.1101/2024.02.19.581004.abstract. Cite Download
Chen, Xinyi, Yapeng Su, Chloe Dai, Jason D. Goldman, James R. Heath, Si Liu, and Wei Sun. 2024. “Association between COVID-19 Disease Severity and T Cell Receptor Repertoire.” bioRxiv. http://doi.org/10.1101/2024.11.26.625333. Cite Download
Kader, Tanjina, Jia-Ren Lin, Clemens B. Hug, Shannon Coy, Yu-An Chen, Ino de Bruijn, Natalie Shih, et al. 2024. “Multimodal Spatial Profiling Reveals Immune Suppression and Microenvironment Remodeling in Fallopian Tube Precursors to High-Grade Serous Ovarian Carcinoma.” Cancer Discovery. http://doi.org/10.1158/2159-8290.CD-24-1366. Cite
Kornilov, Sergey A., Nathan D. Price, Richard Gelinas, Juan Acosta, Mary E. Brunkow, Tiffany Gervasi-Follmar, Ryan C. Winger, et al. 2024. “Multi-Omic Characterization of the Effects of Ocrelizumab in Patients with Relapsing-Remitting Multiple Sclerosis.” Journal of the Neurological Sciences 467: 123303. http://doi.org/10.1016/j.jns.2024.123303. Cite
Matuozzo, Daniela, Estelle Talouarn, Astrid Marchal, Peng Zhang, Jeremy Manry, Yoann Seeleuthner, Yu Zhang, et al. 2024. “Correction: Rare Predicted Loss-of-Function Variants of Type I IFN Immunity Genes Are Associated with Life-Threatening COVID-19.” Genome Medicine 16 (1): 6. http://doi.org/10.1186/s13073-023-01278-0. Cite Download
Armstrong, Sujit Silas, Daniel G. Chen, Sunil Kumar, James R. Heath, Matthew Feinstein, John R. Greenland, Daniel R. Calabrese, Lewis L. Lanier, Klaus Ley, and Avishai Shemesh. 2024. “CITE-Seq Analysis Reveals Human Cytomegalovirus and Diabetes-Associated Adaptive NK Cell Alterations in Cardiovascular Disease.” bioRxiv, 2024–03. https://www.biorxiv.org/content/10.1101/2024.03.22.581997.abstract. Cite Download
Faust, Saul N., Richard Haynes, Christine E. Jones, Natalie Staplin, Elizabeth Whittaker, Thomas Jaki, Ed Juszczak, Enti Spata, Mandy Wan, and James R. Heath. 2024. “Immunomodulatory Therapy in Children with Paediatric Inflammatory Multisystem Syndrome Temporally Associated with SARS-CoV-2 (PIMS-TS, MIS-C; RECOVERY): A Randomised, Controlled, Open-Label, Platform Trial.” The Lancet Child & Adolescent Health 8 (3): 190–200. https://www.thelancet.com/journals/lanchi/article/PIIS2352-4642(23)00316-4/fulltext. Cite
Heath, James R. 2024. “Abstract SY41-02: Experimental Methods for High Throughput Discovery and Validation of Tumor Antigens.” Cancer Research 84 (7_Supplement): SY41-02. https://aacrjournals.org/cancerres/article/84/7_Supplement/SY41-02/742137. Cite
Kim, Jungwoo, Rachel H. Ng, JingXin Liang, Dazy Johnson, Young Shik Shin, Arion F. Chatziioannou, Michael E. Phelps, Wei Wei, Raphael D. Levine, and James R. Heath. 2024. “Kinetic Trajectories of Glucose Uptake in Single Cancer Cells Reveal a Drug-Induced Cell-State Change Within Hours of Drug Treatment.” The Journal of Physical Chemistry. B. http://doi.org/10.1021/acs.jpcb.4c03663. Cite
Nguyen, Kim Truc, Xilal Y. Rima, Luong T. H. Nguyen, Xinyu Wang, Kwang Joo Kwak, Min Jin Yoon, Hong Li, et al. 2024. “Integrated Antigenic and Nucleic Acid Detection in Single Virions and Extracellular Vesicles with Viral Content.” Advanced Healthcare Materials, e2400622. http://doi.org/10.1002/adhm.202400622. Cite
Chen, Daniel G., Jingyi Xie, Jongchan Choi, Rachel H. Ng, Rongyu Zhang, Sarah Li, Rick Edmark, et al. 2024. “Integrative Systems Biology Reveals NKG2A-Biased Immune Responses Correlate with Protection in Infectious Disease, Autoimmune Disease, and Cancer.” Cell Reports 43 (3). http://doi.org/10.1016/j.celrep.2024.113872. Cite Download
Thaweethai, Tanayott, Sarah E. Jolley, Elizabeth W. Karlson, Emily B. Levitan, Bruce Levy, Grace A. McComsey, Lisa McCorkell, et al. 2023. “Development of a Definition of Postacute Sequelae of SARS-CoV-2 Infection.” JAMA 329 (22): 1934–46. http://doi.org/10.1001/jama.2023.8823. Cite Download
Foy, Susan, Cliff Wang, Benjamin Yuen, Sara Said, Boi Quach, Jonathan Hoover, Saparya Nayak, et al. 2023. “Abstract 901: Monitoring of Neoantigens and Adoptively Transferred Personalized neoTCR T Cell Populations in the Tumor and PBMCs of Patients with Solid Tumors.” Cancer Research 83 (7_Supplement): 901. http://doi.org/10.1158/1538-7445.AM2023-901. Cite
Heath, James R., Daniel Chen, Jingyi Xie, Jongchan Choi, Rachel Ng, Rongyu Zhang, Sarah Li, et al. 2023. An NKG2A Biased Immune Response Confers Protection for Infection, Autoimmune Disease, and Cancer. Preprint. In Review. doi:10.21203/rs.3.rs-3413673/v1. Cite Download
Chen, Daniel G., Jingyi Xie, Yapeng Su, and James R. Heath. 2023. “T Cell Receptor Sequences Are the Dominant Factor Contributing to the Phenotype of CD8+ T Cells with Specificities against Immunogenic Viral Antigens.” Cell Reports 42 (11): 113279. http://doi.org/10.1016/j.celrep.2023.113279. Cite
Cheng, Hanjun, Yin Tang, Zhonghan Li, Zhili Guo, James R. Heath, Min Xue, and Wei Wei. 2023. “Non-Mass Spectrometric Targeted Single-Cell Metabolomics.” Trends in Analytical Chemistry: TRAC 168: 117300. http://doi.org/10.1016/j.trac.2023.117300. Cite
Ng, Alphonsus H. C., Huiqian Hu, Kai Wang, Kelsey Scherler, Sarah E. Warren, Daniel R. Zollinger, Jill McKay-Fleisch, et al. 2023. “Organ-Specific Immunity: A Tissue Analysis Framework for Investigating Local Immune Responses to SARS-CoV-2.” Cell Reports 42 (10): 113212. http://doi.org/10.1016/j.celrep.2023.113212. Cite Download
Nag, Arundhati, Amirhossein Mafi, Samir Das, Mary Beth Yu, Belen Alvarez-Villalonga, Soo-Kyung Kim, Yapeng Su, William A. Goddard, and James R. Heath. 2023. “Stereochemical Engineering Yields a Multifunctional Peptide Macrocycle Inhibitor of Akt2 by Fine-Tuning Macrocycle-Cell Membrane Interactions.” Communications Chemistry 6 (1): 95. http://doi.org/10.1038/s42004-023-00890-w. Cite Download
Finton, Kathryn A. K., Peter B. Rupert, Della J. Friend, Ana Dinca, Erica S. Lovelace, Matthew Buerger, Domnita V. Rusnac, et al. 2023. “Effects of HLA Single Chain Trimer Design on Peptide Presentation and Stability.” Frontiers in Immunology 14: 1170462. http://doi.org/10.3389/fimmu.2023.1170462. Cite Download
Flower, Cameron T., Chunmei Liu, James R. Heath, Wei Wei, and Forest M. White. 2023. “Abstract 4876: A Systems Pharmacology Approach to Discover Synergistic Targeted Therapy Combinations.” Cancer Research 83 (7_Supplement): 4876. http://doi.org/10.1158/1538-7445.AM2023-4876. Cite
Chour, William, Jongchan Choi, Jingyi Xie, Mary E. Chaffee, Thomas M. Schmitt, Kathryn Finton, Diana C. DeLucia, et al. 2023. “Large Libraries of Single-Chain Trimer Peptide-MHCs Enable Antigen-Specific CD8+ T Cell Discovery and Analysis.” Communications Biology 6 (1): 528. http://doi.org/10.1038/s42003-023-04899-8. Cite Download
Xu, Alexander M., William Chour, Diana C. DeLucia, Yapeng Su, Ana Jimena Pavlovitch-Bedzyk, Rachel Ng, Yusuf Rasheed, Mark M. Davis, John K. Lee, and James R. Heath. 2023. “Entropic Analysis of Antigen-Specific CDR3 Domains Identifies Essential Binding Motifs Shared by CDR3s with Different Antigen Specificities.” Cell Systems 14 (4): 273-284.e5. http://doi.org/10.1016/j.cels.2023.03.001. Cite
Matuozzo, Daniela, Estelle Talouarn, Astrid Marchal, Peng Zhang, Jeremy Manry, Yoann Seeleuthner, Yu Zhang, et al. 2023. “Rare Predicted Loss-of-Function Variants of Type I IFN Immunity Genes Are Associated with Life-Threatening COVID-19.” Genome Medicine 15 (1): 22. http://doi.org/10.1186/s13073-023-01173-8. Cite Download
Solomon, Benjamin D., Hong Zheng, Laura W. Dillon, Jason D. Goldman, Christopher S. Hourigan, James R. Heath, and Purvesh Khatri. 2023. “Prediction of HLA Genotypes from Single-Cell Transcriptome Data.” Frontiers in Immunology 14: 1146826. http://doi.org/10.3389/fimmu.2023.1146826. Cite Download
Horwitz, Leora I., Tanayott Thaweethai, Shari B. Brosnahan, Mine S. Cicek, Megan L. Fitzgerald, Jason D. Goldman, Rachel Hess, et al. 2023. “Researching COVID to Enhance Recovery (RECOVER) Adult Study Protocol: Rationale, Objectives, and Design.” PloS One 18 (6): e0286297. http://doi.org/10.1371/journal.pone.0286297. Cite Download
Puig-Saus, Cristina, Barbara Sennino, Songming Peng, Clifford L. Wang, Zheng Pan, Benjamin Yuen, Bhamini Purandare, et al. 2023. “Neoantigen-Targeted CD8+ T Cell Responses with PD-1 Blockade Therapy.” Nature. http://doi.org/10.1038/s41586-023-05787-1. Cite
Stawiski, Eric W., Vinnu Bhardwaj, Jyoti Mathur, Eva Huang, Olivier Dalmas, Zheng Pan, Cliff Wang, et al. 2022. “Abstract 2757: Correlates of Peptide-HLA Manufacturing Success, TCR Capture and neoTCR Trafficking from Patients Using the PACTImmune࣪ Database.” Cancer Research 82 (12_Supplement): 2757. http://doi.org/10.1158/1538-7445.AM2022-2757. Cite
Heath, James R., William Chour, Jongchan Choi, Jingyi Xie, Mary Chaffee, Thomas Schmitt, Kathryn Finton, et al. 2022. “Large Libraries of Single-Chain Trimer Peptide-MHCs Enable Rapid Antigen-Specific CD8+ T Cell Discovery and Analysis.” Research Square, rs.3.rs-1090664. http://doi.org/10.21203/rs.3.rs-1090664/v1. Cite Download
Saus, Cristina Puig, Barbara Sennino, Songming Peng, Zheng Pan, Benjamin Yuen, Bhamini Purandare, Kyle Jacoby, et al. 2022. “123 Landscape Analysis of the Neoepitope-Specific T Cell Responses in Patients with and without Clinical Benefit from Immune Checkpoint Blockade Therapy.” Journal for ImmunoTherapy of Cancer 10 (Suppl 2). http://doi.org/10.1136/jitc-2022-SITC2022.0123. Cite Download
Repovic, Pavle, Jongchan Choi, Sunga Hong, Jennifer Reola, Kelly Chun, Christos Petropoulos, James Bowen, et al. 2022. “Temporal Trends in Humoral and Cellular Immune Responses to SARS-CoV-2 mRNA Vaccines Among Multiple Sclerosis Patients Treated With Natalizumab, Ocrelizumab or Fumarates (P14-4.006).” Neurology 98 (18 Supplement). https://n.neurology.org/content/98/18_Supplement/3523. Cite
Manry, Jeremy, Paul Bastard, Adrian Gervais, Tom Le Voyer, Jérémie Rosain, Quentin Philippot, Eleftherios Michailidis, et al. 2022. “The Risk of COVID-19 Death Is Much Greater and Age-Dependent with Type I IFN Autoantibodies (Preprint).” https://pesquisa.bvsalud.org/global-literature-on-novel-coronavirus-2019-ncov/resource/pt/dx.doi.org/10.21203/rs.3.rs-1225906/v1. Cite