Kristian Swearingen, PhD

Principal Scientist

Moritz Lab

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Dr. Swearingen’s research focuses on applying analytical chemistry tools and approaches, especially mass spectrometry, toward understanding the biology of pathogens that affect human health. His current work focuses primarily on parasites of the genus Plasmodium, the causative agent of the disease malaria. Dr. Swearingen uses mass spectrometry to identify and quantify how proteins are expressed and modified in Plasmodium with the aim of identifying new targets for therapies to block invasion and transmission of the parasite.

MS, Analytical Chemistry, University of Alaska Fairbanks, 2005

PhD, Analytical Chemistry, University of Washington, 2010

Proteomics, Mass spectrometry, Plasmodium, Malaria, Ion mobility, FAIMS, Post-translational Modifications

2323737 2RQKSFR5 swearingen 1 chicago-author-date-16th-edition 50 date desc year 1 1 8082 https://isbscience.org/wp-content/plugins/zotpress/

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Rios, Kelly T., James P. McGee, Aswathy Sebastian, Robert L. Moritz, Marina Feric, Sabrina Absalon, Kristian E. Swearingen, and Scott E. Lindner. 2024. “Global Release of Translational Repression Across Plasmodium’s Host-to-Vector Transmission Event.” bioRxiv: The Preprint Server for Biology, 2024.02.01.577866. http://doi.org/10.1101/2024.02.01.577866. Cite Download Download

Reddy, Panga J., Zhi Sun, Helisa H. Wippel, David H. Baxter, Kristian Swearingen, David D. Shteynberg, Mukul K. Midha, et al. 2024. “Borrelia PeptideAtlas: A Proteome Resource of Common Borrelia Burgdorferi Isolates for Lyme Research.” Scientific Data 11 (1): 1313. http://doi.org/10.1038/s41597-024-04047-9. Cite

Nagar, Rupa, Stefano S. Garcia Castillo, Maria Pinzon-Ortiz, Sharon Patray, Alida Coppi, Sachie Kanatani, Robert L. Moritz, Kristian E. Swearingen, Michael A. J. Ferguson, and Photini Sinnis. 2024. “The Major Surface Protein of Malaria Sporozoites Is GPI-Anchored to the Plasma Membrane.” The Journal of Biological Chemistry 300 (8): 107557. http://doi.org/10.1016/j.jbc.2024.107557. Cite

Jennison, Charlie, Janna M. Armstrong, Dorender A. Dankwa, Nina Hertoghs, Sudhir Kumar, Biley A. Abatiyow, Myo Naung, et al. 2023. “Plasmodium GPI-Anchored Micronemal Antigen Is Essential for Parasite Transmission through the Mosquito Host.” Molecular Microbiology. http://doi.org/10.1111/mmi.15078. Cite Download

Jennison, Charlie, Janna M. Gibson, Nina Hertoghs, Dorender A. Dankwa, Sudhir Kumar, Biley A. Abatiyow, Myo Naung, et al. 2022. “Plasmodium GPI-Anchored Micronemal Antigen Is Essential for Parasite Transmission through the Mosquito Host.” bioRxiv. http://doi.org/10.1101/2022.02.24.481744. Cite Download

Lindner, Scott E., Kristian E. Swearingen, Melanie J. Shears, Aswathy Sebastian, Michael P. Walker, Erin N. Vrana, Kevin J. Hart, et al. 2022. “Addendum: Transcriptomics and Proteomics Reveal Two Waves of Translational Repression during the Maturation of Malaria Parasite Sporozoites.” Nature Communications 13 (1): 283. http://doi.org/10.1038/s41467-021-27767-7. Cite Download

Kumar, Sudhir, Meseret T. Haile, Michael R. Hoopmann, Linh T. Tran, Samantha A. Michaels, Seamus R. Morrone, Kayode K. Ojo, et al. 2021. “Plasmodium Falciparum Calcium-Dependent Protein Kinase 4 Is Critical for Male Gametogenesis and Transmission to the Mosquito Vector.” mBio, e0257521. http://doi.org/10.1128/mBio.02575-21. Cite

Steel, Ryan W. J., Vladimir Vigdorovich, Nicholas Dambrauskas, Brandon K. Wilder, Silvia A. Arredondo, Debashree Goswami, Sudhir Kumar, et al. 2021. “Platelet Derived Growth Factor Receptor β (PDGFRβ) Is a Host Receptor for the Human Malaria Parasite Adhesin TRAP.” Scientific Reports 11 (1): 11328. http://doi.org/10.1038/s41598-021-90722-5. Cite Download

Searle, Brian C., Kristian E. Swearingen, Christopher A. Barnes, Tobias Schmidt, Siegfried Gessulat, Bernhard Küster, and Mathias Wilhelm. 2020. “Generating High Quality Libraries for DIA MS with Empirically Corrected Peptide Predictions.” Nature Communications 11 (1): 1548. http://doi.org/10.1038/s41467-020-15346-1. Cite Download

Swearingen, Kristian E., Jimmy K. Eng, David Shteynberg, Vladimir Vigdorovich, Timothy A. Springer, Luis Mendoza, D. Noah Sather, Eric W. Deutsch, Stefan H. I. Kappe, and Robert L. Moritz. 2019. “A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.” Journal of Proteome Research 18 (2): 652–63. http://doi.org/10.1021/acs.jproteome.8b00638. Cite

Lindner, Scott E., Kristian E. Swearingen, Melanie J. Shears, Michael P. Walker, Erin N. Vrana, Kevin J. Hart, Allen M. Minns, Photini Sinnis, Robert L. Moritz, and Stefan H. I. Kappe. 2019. “Transcriptomics and Proteomics Reveal Two Waves of Translational Repression during the Maturation of Malaria Parasite Sporozoites.” Nature Communications 10 (1): 4964. http://doi.org/10.1038/s41467-019-12936-6. Cite

Shears, Melanie J., Raja Sekhar Nirujogi, Kristian E. Swearingen, Santosh Renuse, Satish Mishra, Panga Jaipal Reddy, Robert L. Moritz, Akhilesh Pandey, and Photini Sinnis. 2019. “Proteomic Analysis of Plasmodium Merosomes: The Link between Liver and Blood Stages in Malaria.” Journal of Proteome Research. http://doi.org/10.1021/acs.jproteome.9b00324. Cite

Arredondo, Silvia A., Kristian E. Swearingen, Thomas Martinson, Ryan Steel, Dorender A. Dankwa, Anke Harupa, Nelly Camargo, et al. 2018. “The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes.” Frontiers in Cellular and Infection Microbiology 8: 413. http://doi.org/10.3389/fcimb.2018.00413. Cite Download

Swearingen, Kristian E., and Scott E. Lindner. 2018. “Plasmodium Parasites Viewed through Proteomics.” Trends in Parasitology. http://doi.org/10.1016/j.pt.2018.08.003. Cite

Swearingen, Kristian E., Scott E. Lindner, Erika L. Flannery, Ashley M. Vaughan, Robert D. Morrison, Rapatbhorn Patrapuvich, Cristian Koepfli, et al. 2017. “Proteogenomic Analysis of the Total and Surface-Exposed Proteomes of Plasmodium Vivax Salivary Gland Sporozoites.” PLoS Neglected Tropical Diseases 11 (7): e0005791. http://doi.org/10.1371/journal.pntd.0005791. Cite

Swearingen, Kristian E., Scott E. Lindner, Lirong Shi, Melanie J. Shears, Anke Harupa, Christine S. Hopp, Ashley M. Vaughan, et al. 2016. “Interrogating the Plasmodium Sporozoite Surface: Identification of Surface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP by Mass Spectrometry-Based Proteomics.” PLoS Pathogens 12 (4): e1005606. http://doi.org/10.1371/journal.ppat.1005606. Cite

Swearingen, K. E., J. M. Winget, M. R. Hoopmann, U. Kusebauch, and R. L. Moritz. 2015. “Decreased Gap Width in a Cylindrical High-Field Asymmetric Waveform Ion Mobility Spectrometry Device Improves Protein Discovery.” Analytical Chemistry 87 (December): 12230–37. http://doi.org/10.1021/acs.analchem.5b03199. Cite

Lindner, S. E., K. E. Swearingen, A. Harupa, A. M. Vaughan, P. Sinnis, R. L. Moritz, and S. H. Kappe. 2013. “Total and Putative Surface Proteomics of Malaria Parasite Salivary Gland Sporozoites.” Molecular & Cellular Proteomics : MCP, January. Cite

Swearingen, K. E., and R. L. Moritz. 2012. “High-Field Asymmetric Waveform Ion Mobility Spectrometry for Mass Spectrometry-Based Proteomics.” Expert Review of Proteomics 9 (5): 505–17. Cite

Swearingen, K. E., M. R. Hoopmann, R. S. Johnson, R. A. Saleem, J. D. Aitchison, and R. L. Moritz. 2011. “Nanospray FAIMS Fractionation Provides Significant Increases in Proteome Coverage of Unfractionated Complex Protein Digests.” Molecular & Cellular Proteomics : MCP, December. Cite